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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-6-14
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pubmed:abstractText |
A growth-promoting polypeptide has been purified about 1950-fold from rat abdominal muscle by gel chromatography, isoelectric-focusing (IEF) and reverse phase high pressure liquid chromatographic (RP-HPLC) techniques. This factor is an acidic protein with an isoelectric point (pI) of 3.4 and a mol. wt of 12 kDa when run on SDS-polyacrylamide gels under reducing conditions. It is acid and heat-stable but is sensitive to reducing agent and protease action. It stimulates [3H]thymidine incorporation in NRK-49F and NIH 3T3 cells and induces soft agar colony formation of NIH 3T3 cells. The muscle-derived mitogen appears to differ from other known growth factors (GFs) in its physico-chemical properties.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-34
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2713821-Animals,
pubmed-meshheading:2713821-Binding, Competitive,
pubmed-meshheading:2713821-Cell Line,
pubmed-meshheading:2713821-Cell Transformation, Neoplastic,
pubmed-meshheading:2713821-Cells, Cultured,
pubmed-meshheading:2713821-Chromatography, Gel,
pubmed-meshheading:2713821-Chromatography, High Pressure Liquid,
pubmed-meshheading:2713821-DNA Replication,
pubmed-meshheading:2713821-Female,
pubmed-meshheading:2713821-Isoelectric Focusing,
pubmed-meshheading:2713821-Mitogens,
pubmed-meshheading:2713821-Molecular Weight,
pubmed-meshheading:2713821-Muscles,
pubmed-meshheading:2713821-Proteins,
pubmed-meshheading:2713821-Rats,
pubmed-meshheading:2713821-Rats, Inbred Strains,
pubmed-meshheading:2713821-Receptor, Epidermal Growth Factor
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pubmed:year |
1989
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pubmed:articleTitle |
A mitogenic factor from rat muscle.
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pubmed:affiliation |
Biological Chemistry Division, Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
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pubmed:publicationType |
Journal Article
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