rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
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pubmed:dateCreated |
1989-5-26
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pubmed:abstractText |
Firefly luciferase was immobilized on epoxy methacrylate beads and used for a continuous-flow assay of ATP extracted from platelets. The immobilized luciferase had a half-life of 3 days at 25 degrees C; there was a 25% recovery of luciferase activity upon immobilization, and ca 50 reactors were made from 1 mg of commercial enzyme. The sensitivity of the assay was 0.3 pmol of ATP, and the response was linear between 1 and 500 pmol of ATP. The ATP content of platelets obtained with the present method correlated well with those obtained using soluble luciferase.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:issn |
0884-3996
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
3
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
7-11
|
pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2711891-Adenosine Triphosphate,
pubmed-meshheading:2711891-Animals,
pubmed-meshheading:2711891-Beetles,
pubmed-meshheading:2711891-Blood Platelets,
pubmed-meshheading:2711891-Enzyme Stability,
pubmed-meshheading:2711891-Enzymes, Immobilized,
pubmed-meshheading:2711891-Humans,
pubmed-meshheading:2711891-Hydrogels,
pubmed-meshheading:2711891-Luciferases,
pubmed-meshheading:2711891-Polyhydroxyethyl Methacrylate
|
pubmed:articleTitle |
Continuous-flow bioluminescent determination of ATP in platelets using firefly luciferase immobilized on epoxy methacrylate.
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pubmed:affiliation |
Istituto di Chimica degli Ormoni, CNR, Milano, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|