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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1989-6-5
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pubmed:abstractText |
The lytic effect of complement on human erythrocytes has been reported by others to increase when Na+ is substituted for K+ in the external medium. In this paper we have investigated the hypothesis that net loss of K+ through a K+ transport pathway protects erythrocytes from complement-induced colloidosmotic swelling and lysis. Antibody-sensitized human erythrocytes containing different intracellular cation concentrations (nystatin treatment) were exposed to low concentrations of guinea pig serum in media of different cation composition; complement lysis was assessed by the release of hemoglobin and the volume of the surviving cells estimated by their density distribution profiles. Complement-dependent swelling and lysis of erythrocytes (a) were limited by the presence of an outwardly directed K+ electrochemical gradient and (b) were enhanced by carbocyanine, a specific inhibitor of the Ca2+-activated K+ transport pathway, and by absence of Ca2+ in the external medium. We propose that during complement activation a rising cytosolic calcium triggers the Ca2+-activated K+ permeability pathway, the Gardos effect, produces a net K+, Cl- and water loss, and thus limits the colloidosmotic swelling and lysis of erythrocytes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-1004580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-13618284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-239058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-2439543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-2444891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-2650991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3403718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3518749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3545490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3593311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3711667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3826356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3950577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-3998150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-4014447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-4717992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-5352363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-6171865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-6186685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-6716452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-6808083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-6822660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-7011197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2708520-748181
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1466-71
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2708520-Animals,
pubmed-meshheading:2708520-Biological Transport,
pubmed-meshheading:2708520-Calcium,
pubmed-meshheading:2708520-Cell Membrane Permeability,
pubmed-meshheading:2708520-Colloids,
pubmed-meshheading:2708520-Complement Activation,
pubmed-meshheading:2708520-Electrochemistry,
pubmed-meshheading:2708520-Erythrocyte Membrane,
pubmed-meshheading:2708520-Guinea Pigs,
pubmed-meshheading:2708520-Hemolysis,
pubmed-meshheading:2708520-Humans,
pubmed-meshheading:2708520-Hypotonic Solutions,
pubmed-meshheading:2708520-Osmotic Pressure,
pubmed-meshheading:2708520-Potassium Channels,
pubmed-meshheading:2708520-Potassium Chloride,
pubmed-meshheading:2708520-Sodium Chloride
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pubmed:year |
1989
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pubmed:articleTitle |
Ca2+-activated K+ efflux limits complement-mediated lysis of human erythrocytes.
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pubmed:affiliation |
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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