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pubmed-article:2708289pubmed:abstractTextAn ion-exchange chromatographic method was developed to determine hypoglycin A (HG-A) levels in canned ackee fruit by using an amino acid analyzer. HG-A was extracted by homogenizing the sample in 80% alcohol. An isocratic buffer system, consisting of 30% sodium citrate buffer (pH 3.15) and 70% sodium chloride-sodium acetate buffer (pH 7.40) was used to obtain baseline separation between HG-A and the other amino acids. The system can detect HG-A levels as low as 4.8 micrograms/mL. HG-A levels in the edible portion of fruit in 6 cans ranged from 11.0 to 66.5 mg HG-A/can. Recoveries by standard addition averaged 102.5%.lld:pubmed
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pubmed-article:2708289pubmed:authorpubmed-author:ChangE HEHlld:pubmed
pubmed-article:2708289pubmed:authorpubmed-author:LandenW OWOJrlld:pubmed
pubmed-article:2708289pubmed:authorpubmed-author:SolimanA GAGlld:pubmed
pubmed-article:2708289pubmed:authorpubmed-author:GelbaumL TLTlld:pubmed
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pubmed-article:2708289pubmed:dateRevised2001-3-23lld:pubmed
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pubmed-article:2708289pubmed:articleTitleIon-exchange chromatographic determination of hypoglycin A in canned ackee fruit.lld:pubmed
pubmed-article:2708289pubmed:affiliationFood and Drug Administration, Atlanta, GA 30309.lld:pubmed
pubmed-article:2708289pubmed:publicationTypeJournal Articlelld:pubmed