rdf:type |
|
lifeskim:mentions |
umls-concept:C0035696,
umls-concept:C0043342,
umls-concept:C0059036,
umls-concept:C0205164,
umls-concept:C0300140,
umls-concept:C0679058,
umls-concept:C1519595,
umls-concept:C1547699,
umls-concept:C1705165,
umls-concept:C2003941,
umls-concept:C2700061,
umls-concept:C2700640
|
pubmed:issue |
1
|
pubmed:dateCreated |
1989-6-2
|
pubmed:databankReference |
|
pubmed:abstractText |
A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-alpha (EF-1 alpha) is synthesized in very large amounts in the early embryo. Transcription of EF-1 alpha mRNA commences at the midblastula transition (MBT), and new EF-1 alpha protein is synthesized very soon after this, as determined by the association of EF-1 alpha mRNA with polysomes. The nucleotide sequence of a full-length EF-1 alpha cDNA clone and the deduced amino acid sequence of Xenopus EF-1 alpha protein are presented.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0012-1606
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
133
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
93-100
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:2707491-Amino Acid Sequence,
pubmed-meshheading:2707491-Animals,
pubmed-meshheading:2707491-Base Sequence,
pubmed-meshheading:2707491-Blastocyst,
pubmed-meshheading:2707491-DNA,
pubmed-meshheading:2707491-DNA Probes,
pubmed-meshheading:2707491-Molecular Sequence Data,
pubmed-meshheading:2707491-Nucleic Acid Hybridization,
pubmed-meshheading:2707491-Peptide Elongation Factor 1,
pubmed-meshheading:2707491-Peptide Elongation Factors,
pubmed-meshheading:2707491-Polyribosomes,
pubmed-meshheading:2707491-RNA, Messenger,
pubmed-meshheading:2707491-RNA Probes,
pubmed-meshheading:2707491-Transcription, Genetic,
pubmed-meshheading:2707491-Xenopus laevis
|
pubmed:year |
1989
|
pubmed:articleTitle |
The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
|
pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|