Linked Life Data

2706860

Source:http://linkedlifedata.com/resource/pubmed/id/2706860

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
242
pubmed:dateCreated
1989-5-31
pubmed:abstractText
Isolated human chondrocytes derived from healthy and osteoarthritic (OA) cartilage were cultured in high density in a newly designed microculture system. The severity of OA was graded according to a modified histopathologic score originally described by Mankin et al. Chondrocytes from adult patients with OA showed 35S-sulphate and 3H-thymidine incorporation in vitro, which increased with severity of the disease through Mankin 11-12. Incorporation rapidly declined after Mankin 11-12. Both matrix synthesis and cell proliferation were strongly reduced in the severe grades of OA. Histologic examination of the newly formed cartilage was only possible if the chondrocytes were derived from less severe grades of OA. Microscopy showed healthy chondrocytes surrounded by newly synthesized matrix, which stained well with specific dyes, indicating the ability of the cells to synthesize normal matrix components. The phenotype of human articular chondrocytes, derived from different grades of OA, was maintained in a high-density culture system. The data suggest dysregulation of the cell metabolism in OA cartilage. The increased cell metabolism was directly related to the histopathologic grade of OA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0009-921X
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
294-302
pubmed:dateRevised
2005-3-3
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Metabolic characteristics of in vitro cultured human chondrocytes in relation to the histopathologic grade of osteoarthritis.
pubmed:affiliation
Department of Orthopaedic Surgery, University of Limburg, The Netherlands.
pubmed:publicationType
Journal Article