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rdf:type |
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lifeskim:mentions |
umls-concept:C0001443,
umls-concept:C0013710,
umls-concept:C0021528,
umls-concept:C0035693,
umls-concept:C0043342,
umls-concept:C0439836,
umls-concept:C0441655,
umls-concept:C0678594,
umls-concept:C1512977,
umls-concept:C1515926,
umls-concept:C1704970
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pubmed:issue |
8
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pubmed:dateCreated |
1989-5-24
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pubmed:abstractText |
Amphibian eggs and embryos as well as mammalian cells have been reported to contain an activity that unwinds double-stranded RNA. We have now found that adenosine residues have been modified in the RNA products of this unwinding activity. Although the modified RNA remains double-stranded, the modification causes the RNA to be susceptible to single-strand-specific RNase and to migrate as a retarded smear on a native polyacrylamide electrophoresis gel. The modification is specific for double-stranded RNA. At least 40% of the adenosine residues can be modified in vitro in a given random sequence RNA molecule. By using standard two-dimensional TLC and HPLC analyses, the modified base has been identified as inosine. Mismatched base-pairing between inosine and uridine appears to be responsible for the observed characteristics of the unwound RNA. The biological significance of this modifying activity and also of the modified double-stranded RNA is discussed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-220499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2427015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2434240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2434241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2451125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2452696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2452697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2874494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-2874495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-3024965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-3167982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-3203381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-3520340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-3621347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-3659919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-4071043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-4557059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-6118863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-6190418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-6200602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-6933441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2704740-7196457
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2647-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
A double-stranded RNA unwinding activity introduces structural alterations by means of adenosine to inosine conversions in mammalian cells and Xenopus eggs.
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pubmed:affiliation |
Wistar Institute, Philadelphia, PA 19104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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