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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1990-5-29
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pubmed:abstractText |
The herbicide suphometuron methyl inhibits the utilization of pyruvate and 2-ketobutyrate by the branched-chain amino acid biosynthetic enzyme acetolactate synthase. Eighteen insertions of the transposon Tn10 into the genome of Salmonella typhimurium LT2 caused hypersensitivity to this herbicide. Five of these insertions conferred a partial auxotrophic requirement. Concurrent herbicide sensitivity and heat-labile pantothenate auxotrophy was due to panD::Tn10 mutations, while coincident sulphometuron methyl sensitivity and thiamin auxotrophy was attributable to thiA::Tn10 mutations. The phenotypes of these mutations suggested that coenzyme A and thiamin pyrophosphate availability modulated the cells' response to sulphometuron methyl. A model suggesting a key role for 2-ketobutyrate accumulation in herbicide action is supported by the function of thiamin pyrophosphate in 2-ketoacid metabolism and the known role of a 2-ketoacid in coenzyme A synthesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1287
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2209-22
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2699328-Acetolactate Synthase,
pubmed-meshheading:2699328-Alleles,
pubmed-meshheading:2699328-Bacterial Proteins,
pubmed-meshheading:2699328-Butyric Acids,
pubmed-meshheading:2699328-Mutation,
pubmed-meshheading:2699328-Oxo-Acid-Lyases,
pubmed-meshheading:2699328-Pantothenic Acid,
pubmed-meshheading:2699328-Phenotype,
pubmed-meshheading:2699328-Pyruvates,
pubmed-meshheading:2699328-Salmonella typhimurium,
pubmed-meshheading:2699328-Sulfonylurea Compounds,
pubmed-meshheading:2699328-Suppression, Genetic,
pubmed-meshheading:2699328-Thiamine
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pubmed:year |
1989
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pubmed:articleTitle |
Leaky pantothenate and thiamin mutations of Salmonella typhimurium conferring suphometuron methyl sensitivity.
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pubmed:affiliation |
Central Research and Development Department, E. I. du Pont de Nemours and Company, Wilmington, DE 19880-0328.
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pubmed:publicationType |
Journal Article
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