| pubmed-article:2698302 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0080103 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0021670 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:2698302 | lifeskim:mentions | umls-concept:C0124026 | lld:lifeskim |
| pubmed-article:2698302 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2698302 | pubmed:dateCreated | 1990-5-4 | lld:pubmed |
| pubmed-article:2698302 | pubmed:abstractText | Titre of islet cell surface antibodies (ICSA) in 114 sera from healthy control probands and 177 sera from first-degree relatives of Type 1 diabetic patients was determined by indirect immunofluorescence using rat insulinoma (RIN) cells as target. All sera were tested at four dilutions (1/40-1/320). 10(5) RIN cells were incubated with 100 microliters diluted serum overnight at 4 degrees C followed by a 45 min-incubation with a FITC-labelled goat anti-human globulin. Titre curves were calculated by double logarithmic regression. ICSA titre was defined as the serum dilution producing cell surface fluorescence on 40% of RIN cells. Based on these data a serum is defined as ICSA positive when the ICSA titre calculated is higher than 1:142, quantil Q (0.97). Twenty-five out of 177 (14%) sera of first-degree relatives of Type 1 diabetes were ICSA positive with a mean titre of 1/393, range 1/145-1/1,740, while 2/114 (1.7%) control sera were weakly positive for ICSA. These data demonstrate the significantly increased ICSA prevalence in first-degree relatives of Type 1 diabetic patients. The present study suggests that RIN cells may represent a useful tool for standardization of ICSA assay. | lld:pubmed |
| pubmed-article:2698302 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2698302 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2698302 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2698302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2698302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2698302 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2698302 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2698302 | pubmed:issn | 0265-5985 | lld:pubmed |
| pubmed-article:2698302 | pubmed:author | pubmed-author:ZieglerMM | lld:pubmed |
| pubmed-article:2698302 | pubmed:author | pubmed-author:ZieglerBB | lld:pubmed |
| pubmed-article:2698302 | pubmed:author | pubmed-author:HeinkePP | lld:pubmed |
| pubmed-article:2698302 | pubmed:author | pubmed-author:MichaelisDD | lld:pubmed |
| pubmed-article:2698302 | pubmed:author | pubmed-author:RjasanowskiII | lld:pubmed |
| pubmed-article:2698302 | pubmed:author | pubmed-author:SchlosserMM | lld:pubmed |
| pubmed-article:2698302 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2698302 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:2698302 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2698302 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2698302 | pubmed:pagination | 25-9 | lld:pubmed |
| pubmed-article:2698302 | pubmed:dateRevised | 2007-9-11 | lld:pubmed |
| pubmed-article:2698302 | pubmed:meshHeading | pubmed-meshheading:2698302-... | lld:pubmed |
| pubmed-article:2698302 | pubmed:meshHeading | pubmed-meshheading:2698302-... | lld:pubmed |
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| pubmed-article:2698302 | pubmed:meshHeading | pubmed-meshheading:2698302-... | lld:pubmed |
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| pubmed-article:2698302 | pubmed:meshHeading | pubmed-meshheading:2698302-... | lld:pubmed |
| pubmed-article:2698302 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2698302 | pubmed:articleTitle | Determination of islet cell surface antibodies in first-degree relatives of type 1 diabetic patients using rat insulinoma cells. | lld:pubmed |
| pubmed-article:2698302 | pubmed:affiliation | Central Institute of Diabetes Gerhardt Katsch, Karlsburg, GDR. | lld:pubmed |
| pubmed-article:2698302 | pubmed:publicationType | Journal Article | lld:pubmed |
