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| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C0237753 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C1285572 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C1314763 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C0449851 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C0079809 | lld:lifeskim |
| pubmed-article:2698190 | lifeskim:mentions | umls-concept:C1283195 | lld:lifeskim |
| pubmed-article:2698190 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2698190 | pubmed:dateCreated | 1990-5-1 | lld:pubmed |
| pubmed-article:2698190 | pubmed:abstractText | A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In this study, the technique has been applied to the analysis of cytokines produced by lymphoid cells ranging in number from a single cell to 10(6) cells. The technique should be applicable to virtually any tissue or cell type. | lld:pubmed |
| pubmed-article:2698190 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2698190 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2698190 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2698190 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2698190 | pubmed:issn | 0736-6205 | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:SuzukiNN | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:EnglemanE GEG | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:LarrickJ WJW | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:FryK EKE | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:NelsonP APA | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:RaoC UCU | lld:pubmed |
| pubmed-article:2698190 | pubmed:author | pubmed-author:BrennerC ACA | lld:pubmed |
| pubmed-article:2698190 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2698190 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:2698190 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2698190 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2698190 | pubmed:pagination | 1096-103 | lld:pubmed |
| pubmed-article:2698190 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
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| pubmed-article:2698190 | pubmed:articleTitle | Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells. | lld:pubmed |
| pubmed-article:2698190 | pubmed:affiliation | Genelabs Inc., Redwood City, CA 94063. | lld:pubmed |
| pubmed-article:2698190 | pubmed:publicationType | Journal Article | lld:pubmed |
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