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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1990-5-1
|
| pubmed:abstractText |
A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In this study, the technique has been applied to the analysis of cytokines produced by lymphoid cells ranging in number from a single cell to 10(6) cells. The technique should be applicable to virtually any tissue or cell type.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1096-103
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2698190-Base Sequence,
pubmed-meshheading:2698190-Biological Factors,
pubmed-meshheading:2698190-Biotechnology,
pubmed-meshheading:2698190-Cell Line,
pubmed-meshheading:2698190-Cytokines,
pubmed-meshheading:2698190-DNA,
pubmed-meshheading:2698190-Genetic Techniques,
pubmed-meshheading:2698190-Humans,
pubmed-meshheading:2698190-Kinetics,
pubmed-meshheading:2698190-Molecular Sequence Data,
pubmed-meshheading:2698190-Phenotype,
pubmed-meshheading:2698190-Polymerase Chain Reaction,
pubmed-meshheading:2698190-RNA, Messenger
|
| pubmed:articleTitle |
Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.
|
| pubmed:affiliation |
Genelabs Inc., Redwood City, CA 94063.
|
| pubmed:publicationType |
Journal Article
|