Linked Life Data

2693944

Source:http://linkedlifedata.com/resource/pubmed/id/2693944

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1990-3-15
pubmed:abstractText
Escherichia coli mutT strains are strong mutators yielding only A.T----C.G transversion mutations. These are thought to result from uncorrected (or unprevented) A.G mispairings during DNA replication. We have investigated the interaction of mutT-induced replication errors with the mutHLS-encoded postreplicative mismatch repair system. By measuring mutation frequencies in both forward and reversion systems, we have demonstrated that mutTmutL and mutTmutS double mutators produce no more mutants than expected from simple additivity of the frequencies in the single mutators. We conclude that mutT-induced A.G replication errors are not recognized by the MutHLS system. On the other hand, direct measurements of mismatch repair by transfection of bacteriophage M13mp2 heteroduplex DNA as well as mutational data from strains other than muT, indicate that the MutHLS system can repair at least certain A.G mispairs. We suggest that A.G mispairs may exist in several different conformations, some of which are recognized by the MutHLS system. However, the A.G mispairs normally prevented by the mutT function are refractory to mismatch repair, indicating that they may represent a structurally distinct class.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
219
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
256-62
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A.T----C.G transversions and their prevention by the Escherichia coli mutT and mutHLS pathways.
pubmed:affiliation
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.