Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6 Pt 2
|
| pubmed:dateCreated |
1990-2-2
|
| pubmed:abstractText |
Because inflammatory processes in renal glomeruli may involve monocyte-macrophages (MPs) and T-lymphocytes, we have investigated whether products of glomerular mesangial cells (MCs) can stimulate the proliferative activity of these effector cells. We found that cultured rat MCs (subcultures 2-15), maintained under serum-free conditions, secrete a soluble factor into the supernate [MC-conditioned medium (CM)], which supports growth of the T-helper cell-derived line HT-2. Moreover, MC-CM increased [3H]thymidine incorporation by thioglycollate-elicited peritoneal MPs but did not induce growth of the interleukin 2 (IL-2)- or interleukin 4 (IL-4)-dependent cell line CTLL-2. Further functional, serological, and biochemical analysis of MC-CM revealed that rat MCs secrete a cytokine that, by all of the techniques used, is indistinguishable from granulocyte-macrophage colony-stimulating factor (GM-CSF). Both northern blot and in situ hybridization with a specific cDNA probe for murine GM-CSF showed that MCs express GM-CSF mRNA transcripts. The present findings indicate that cultured rat MCs produce GM-CSF. Release of GM-CSF by MCs in vivo may play a role in the interaction of MCs with MPs, T-cells, and neutrophils in glomerular disease.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
257
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
F1065-78
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2690641-Animals,
pubmed-meshheading:2690641-B-Lymphocytes,
pubmed-meshheading:2690641-Cell Division,
pubmed-meshheading:2690641-Cell Line,
pubmed-meshheading:2690641-Cells, Cultured,
pubmed-meshheading:2690641-Colony-Stimulating Factors,
pubmed-meshheading:2690641-DNA Replication,
pubmed-meshheading:2690641-Glomerular Mesangium,
pubmed-meshheading:2690641-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2690641-Growth Substances,
pubmed-meshheading:2690641-Humans,
pubmed-meshheading:2690641-Kinetics,
pubmed-meshheading:2690641-Macrophages,
pubmed-meshheading:2690641-Male,
pubmed-meshheading:2690641-Rats,
pubmed-meshheading:2690641-Rats, Inbred Strains,
pubmed-meshheading:2690641-Recombinant Proteins,
pubmed-meshheading:2690641-T-Lymphocytes
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Rat mesangial cells produce granulocyte-macrophage colony-stimulating factor.
|
| pubmed:affiliation |
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|