Linked Life Data

2689885

Source:http://linkedlifedata.com/resource/pubmed/id/2689885

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6251
pubmed:dateCreated
1990-2-1
pubmed:abstractText
Transcription of the HO gene of Saccharomyces cerevisiae, which encodes a site-specific endonuclease that initiates cell-type switching (reviewed in refs. 1,2), is restricted to a short window of the cell cycle in late G1 (refs 3,4). A repeated element in the upstream region of HO (the cell-cycle box, CCB) and two regulatory proteins, SWI4 and SWI6, are required for cell-cycle-dependent expression of HO. Biochemical experiments have identified a factor, CCBF (cell-cycle box factor), that binds to the CCB elements and that presumably plays a key part in cell-cycle regulation of HO. The SWI4 and SWI6 genes are required for formation of the CCBF-DNA complex. Here we report the nucleotide sequence of the SWI4 gene and show that it contains two copies of the conserved SWI6-cdc10 motif observed in SWI6 of budding yeast, the Schizosaccharomyces pombe cdc10 gene required for progression through G19, the Drosophila Notch gene, and in the Caenorhabditis elegans lin-12 and glp-1 genes. We demonstrate by using antibodies to the SWI4 protein in gel-shift assays that the protein is present in the CCBF-DNA complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
342
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
830-3
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription.
pubmed:affiliation
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't