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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1990-2-7
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pubmed:databankReference |
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pubmed:abstractText |
The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1115-25
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2688896-Amino Acid Sequence,
pubmed-meshheading:2688896-Animals,
pubmed-meshheading:2688896-Avian myeloblastosis virus,
pubmed-meshheading:2688896-Base Sequence,
pubmed-meshheading:2688896-Bone Marrow Cells,
pubmed-meshheading:2688896-Cells, Cultured,
pubmed-meshheading:2688896-Chickens,
pubmed-meshheading:2688896-Cloning, Molecular,
pubmed-meshheading:2688896-Deoxyribonuclease I,
pubmed-meshheading:2688896-Escherichia coli,
pubmed-meshheading:2688896-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:2688896-Gene Library,
pubmed-meshheading:2688896-Genes, Regulator,
pubmed-meshheading:2688896-Molecular Sequence Data,
pubmed-meshheading:2688896-Oncogene Proteins v-myb,
pubmed-meshheading:2688896-Oncogenes,
pubmed-meshheading:2688896-Promoter Regions, Genetic,
pubmed-meshheading:2688896-Protein Binding,
pubmed-meshheading:2688896-Protein-Tyrosine Kinases,
pubmed-meshheading:2688896-RNA, Messenger,
pubmed-meshheading:2688896-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2688896-Restriction Mapping,
pubmed-meshheading:2688896-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:2688896-Transcriptional Activation
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pubmed:year |
1989
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pubmed:articleTitle |
The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene.
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pubmed:affiliation |
Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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