Linked Life Data

2687298

Source:http://linkedlifedata.com/resource/pubmed/id/2687298

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-1-24
pubmed:abstractText
In primary cultures of rat preadipocytes (PA) isolated from epididymal or perirenal depots, rat serum is more effective than other animal sera (fetal calf, newborn calf, human, horse, rabbit, cat, sheep, goat, dog, pig) in promoting adipogenic conversion, biochemical differentiation, and mitogenesis. Only mouse serum is comparable to rat serum. This activity is attributable to a specific growth factor (preadipocyte stimulating factor, PSF). An assay for PSF in rat serum was devised using PA from perirenal fat of 3-month-old Fischer 344 rats grown first to confluence in FCS for 8 days and then for the next 3 days in test serum, followed by measurement of triglyceride (TG) and glycerol-3-phosphate dehydrogenase (GPDH). Rat serum induces dose-dependent rapid cell division, which coincides with accumulation of TG and increase of GPDH; for routine quantitation, TG is assayed. The biochemical characteristics of PSF in serum are as follows: stable at 4 degrees C for up to 1 year; inactivated at 100 degrees C (80% loss, 30 min) but stable at 56 degrees C for 1 hr; stable at pH 2-12; non-dialyzable; completely resistant to pepsin, trypsin, and chymotrypsin but destroyed by pronase and subtilisn; stable to DTT and periodate; and m.w. between 68 kDa (Sephacryl-300) and 58 kDa (Sephacryl-300 in 5 M urea). PSF activity is greater in serum from Wistar than from Fischer 344 rats, while activity of serum from Zucker obese (fa/fa) rats is at least as great as that from Wistar rats and, like serum of rats made obese by feeding a high-fat, high-carbohydrate diet, is not suppressed. PSF activity is not due to insulin, insulin-like growth factor-1 (IGF-1), growth hormone, glucocorticoids, or combinations of these hormones. PSF activity was not seen with a number of growth factors including colony-stimulating factor (CSF-1), GM-CSF, interleukins 1, 2, and 3, neuroleukin, tumor necrosis factor, and others. PSF is distinct from the low molecular weight (4-8 kDa) differentiation factor present in rat serum, FCS, and human serum that promotes the adipogenic conversion and cellular differentiation of 3T3-L1, 3T3-F442A, and Ob17 cells. PSF appears to be a new differentiation factor for rat preadipocytes, has properties suggestive of a highly glycosylated protein, and may be highly species specific.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
543-57
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:2687298-Adipose Tissue, pubmed-meshheading:2687298-Animals, pubmed-meshheading:2687298-Blood Proteins, pubmed-meshheading:2687298-Cell Differentiation, pubmed-meshheading:2687298-Cell Division, pubmed-meshheading:2687298-Cells, Cultured, pubmed-meshheading:2687298-Dexamethasone, pubmed-meshheading:2687298-Female, pubmed-meshheading:2687298-Glycerolphosphate Dehydrogenase, pubmed-meshheading:2687298-Growth Hormone, pubmed-meshheading:2687298-Humans, pubmed-meshheading:2687298-Insulin, pubmed-meshheading:2687298-Insulin-Like Growth Factor I, pubmed-meshheading:2687298-Lipid Metabolism, pubmed-meshheading:2687298-Male, pubmed-meshheading:2687298-Molecular Weight, pubmed-meshheading:2687298-Obesity, pubmed-meshheading:2687298-Rats, pubmed-meshheading:2687298-Rats, Inbred F344, pubmed-meshheading:2687298-Triglycerides
pubmed:year
1989
pubmed:articleTitle
Preadipocyte stimulating factor in rat serum: evidence for a discrete 63 kDa protein that promotes cell differentiation of rat preadipocytes in primary cultures.
pubmed:affiliation
Endocrinology Section, National Institute on Aging, Baltimore, Maryland.
pubmed:publicationType
Journal Article, Comparative Study