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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1977-10-14
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pubmed:abstractText |
We have found a unique deoxyribonuclease in extracts of the eukaryotic green alga Chlamydomonas. When incubated with viral DNA from adenovirus-2, this enzyme produces discrete fragments that form bands upon electrophoresis in an agarose gel. Site specificity of the enzymatic cleavage examined by identifying the 5'-terminal nucleotides in cleaved adenovirus-2 DNA and by studies with synthetic polynucleotides of defined sequence, indicates that the initial endonucleolytic cleavage occurs at a site containing a deoxythymidine residue. Electron microscopy of cleaved adenovirus-2 DNA revealed single-strand segments within duplex DNA. We propose that the enzyme acts by making initial site-specific single-strand incisions, followed by subsequent excision on the same strand, producing a gapped duplex molecule; and that double-strand scissions result from limited occurrence of overlapping single-strand gaps on complementary strands.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1054510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1094910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1098045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1098145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1111098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-1190452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-13139273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-17248716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-17248717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-17248718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4287053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4352720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4354250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4506760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4567334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4734785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-4902612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-5323016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-5325727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/268618-810727
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2687-91
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
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pubmed:year |
1977
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pubmed:articleTitle |
A site-specific single-strand endonuclease from the eukaryote Chlamydomonas.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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