Linked Life Data

2683863

Source:http://linkedlifedata.com/resource/pubmed/id/2683863

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-12-12
pubmed:abstractText
We present here a preparation protocol and molecular characterization of mitotic cytoskeletons isolated from embryos of the spiny red sea urchin, Strongylocentrotus franciscanus. The mitotic cytoskeletons are produced by detergent lysis of S. franciscanus embryos during cell division and consist of a mitotic apparatus enclosed in a thin shell of cortical filaments. Mitotic cytoskeletons produced in this way are stable for a year at liquid nitrogen temperature. S. franciscanus is of interest because it is commercially available in large quantities and each animal provides prodigious amounts of gametes. Furthermore, embryos from this sea urchin will develop synchronously at high densities and are an excellent source of quantities of cytoskeletal proteins appropriate for biochemical studies.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Preparation and characterization of mitotic cytoskeletons from embryos of the sea urchin Strongylocentrotus franciscanus.
pubmed:affiliation
Department of Biological Sciences, University of California, Santa Barbara 93106.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.