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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1989-12-5
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pubmed:abstractText |
Although significant progress has been made regarding the structure and function of titin, little data exist on the biosynthesis of this large protein in developing muscle. Using pulse-labeling with [35S]methionine and immunoprecipitation with an anti-titin mAb, we have examined the biosynthesis of titin in synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos. We find that: (a) titin synthesis increases greater than 4-fold during the first week in culture and during this same time period, synthesis of muscle-specific myosin heavy chain increases greater than 12-fold; (b) newly synthesized titin has a t1/2 of approximately 70 h; (c) titin is resistant to extraction with Triton X-100 both during and immediately after its synthesis. These observations suggest that newly synthesized titin molecules are stable proteins that rapidly associate with the cytoskeleton of developing myotubes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-13860529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-2423529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-2453516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3047149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3182948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3342063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3391266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3474634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3476939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3512578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3519219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3536962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3541537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-368807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3724060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3754864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3755803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3888377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3905821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-3905857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-4299101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-4326772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-440082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-4624122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-6384237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-6512859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-6547565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-6587383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-6682861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-6896900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-7095255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-7287636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-7287670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2681227-837445
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2189-95
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2681227-Animals,
pubmed-meshheading:2681227-Antibodies, Monoclonal,
pubmed-meshheading:2681227-Cells, Cultured,
pubmed-meshheading:2681227-Chickens,
pubmed-meshheading:2681227-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2681227-Fluorescent Antibody Technique,
pubmed-meshheading:2681227-Immunoblotting,
pubmed-meshheading:2681227-Kinetics,
pubmed-meshheading:2681227-Membrane Proteins,
pubmed-meshheading:2681227-Methionine,
pubmed-meshheading:2681227-Muscle Proteins,
pubmed-meshheading:2681227-Muscles,
pubmed-meshheading:2681227-Protein Kinases,
pubmed-meshheading:2681227-Sulfur Radioisotopes
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pubmed:year |
1989
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pubmed:articleTitle |
Biosynthesis of titin in cultured skeletal muscle cells.
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pubmed:affiliation |
Department of Biochemistry, University of Iowa, Iowa City 52242.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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