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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-12-5
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pubmed:abstractText |
Fresh lymph-borne (veiled) dendritic cells (L-DC) in the rat are almost totally negative for the interleukin-2 (IL-2) receptor detected by the monoclonal antibody (mAb) MRC OX39. After 16 hr culture more than 90% of L-DC are OX39 positive, and increased levels of expression can be seen within 5 hr culture. In cultures of L-DC and allogeneic lymphocytes. L-DC appear to express the IL-2 receptor more rapidly than lymphocytes. The intensity of labelling of L-DC is variable but maximal levels are similar to those seen on lymphoblasts. Culture in the presence of concanavalin A (Con A)-stimulated spleen cell supernatants or recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) results in a more rapid and intense expression of the IL-2 receptor by L-DC. L-DC cultured following rigorous T-cell depletion, or derived from athymic rats also express the IL-2 receptor after culture with GM-CSF. Cultured, but not fresh, L-DC bind iodinated recombinant IL-2 in a dose-dependent manner and binding is inhibited by excess unlabelled ligand. The amount of IL-2 bound varies but maximal amounts are similar to those bound by lymphoblasts. Following intravenous endotoxin injection, a large proportion of freshly collected L-DC express the IL-2 receptor and the number of L-DC released into the lymph is increased. An antibody to the IL-2 receptor which blocks an allogeneic MLR has no effect on a xenogeneic MLR using rat L-DC as stimulators and mouse lymphocytes as responders.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-2422044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-2423881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-2680906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-2821110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-2828930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3005464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3031660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3039002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3081639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3081898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3086446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3095922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3097144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3100957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3108674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3110786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3340823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3925347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3928803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-3930598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2680907-6142524
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
108-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2680907-Animals,
pubmed-meshheading:2680907-Cells, Cultured,
pubmed-meshheading:2680907-Colony-Stimulating Factors,
pubmed-meshheading:2680907-Dendritic Cells,
pubmed-meshheading:2680907-Endotoxins,
pubmed-meshheading:2680907-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2680907-Growth Substances,
pubmed-meshheading:2680907-Lymph,
pubmed-meshheading:2680907-Rats,
pubmed-meshheading:2680907-Receptors, Interleukin-2,
pubmed-meshheading:2680907-Recombinant Proteins
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pubmed:year |
1989
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pubmed:articleTitle |
Properties of lymph-borne (veiled) dendritic cells in culture. II. Expression of the IL-2 receptor: role of GM-CSF.
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pubmed:affiliation |
Sir William Dunn School of Pathology, Oxford, U.K.
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pubmed:publicationType |
Journal Article
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