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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
1989-11-9
|
| pubmed:abstractText |
A sequence encoding bovine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been identified from a concanavalin A-stimulated bovine lymphocyte cDNA library. This sequence was isolated by hybridization with synthetic oligonucleotide probes based upon the human GM-CSF sequence. This bovine cDNA was engineered for expression and secretion of activity into the periplasmic space of E. coli. Periplasmic extracts contain a 14,500-dalton protein and stimulate colony formation of bovine bone marrow progenitor cells. The predicted protein is 70% homologous with human GM-CSF and 55% homologous with murine GM-CSF. Numerous structural features are conserved among these three proteins, such as location of cysteine residues, glycosylation sites, and overall change. The biological activity of bovine GM-CSF is species specific, since recombinant preparations do not cause proliferation of human or murine bone marrow cells. Similarly, murine GM-CSF does not exhibit activity on cells of bovine or human origin. However, human GM-CSF does stimulate colony formation of bovine bone marrow cells, although the specific activity appears reduced when compared to assays on human cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0165-2427
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
261-78
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2678728-Amino Acid Sequence,
pubmed-meshheading:2678728-Animals,
pubmed-meshheading:2678728-Base Sequence,
pubmed-meshheading:2678728-Cattle,
pubmed-meshheading:2678728-Cloning, Molecular,
pubmed-meshheading:2678728-Colony-Forming Units Assay,
pubmed-meshheading:2678728-Colony-Stimulating Factors,
pubmed-meshheading:2678728-DNA,
pubmed-meshheading:2678728-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2678728-Escherichia coli,
pubmed-meshheading:2678728-Gene Library,
pubmed-meshheading:2678728-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2678728-Growth Substances,
pubmed-meshheading:2678728-Immunoblotting,
pubmed-meshheading:2678728-Mice,
pubmed-meshheading:2678728-Molecular Sequence Data,
pubmed-meshheading:2678728-Plasmids,
pubmed-meshheading:2678728-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2678728-Species Specificity
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Cloning and expression of the cDNA for bovine granulocyte-macrophage colony-stimulating factor.
|
| pubmed:affiliation |
Department of Developmental Biology, South San Francisco, CA 94080.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|