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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1989-11-8
|
| pubmed:abstractText |
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0887-3585
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
266-70
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2678097-Crystallization,
pubmed-meshheading:2678097-DNA-Directed RNA Polymerases,
pubmed-meshheading:2678097-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2678097-Escherichia coli,
pubmed-meshheading:2678097-Molecular Weight,
pubmed-meshheading:2678097-Protein Conformation,
pubmed-meshheading:2678097-T-Phages,
pubmed-meshheading:2678097-X-Ray Diffraction
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Single crystals of bacteriophage T7 RNA polymerase.
|
| pubmed:affiliation |
Department of Crystallography, University of Pittsburgh, Pennsylvania 15260.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|