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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1989-10-28
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pubmed:abstractText |
Plasmid pUN835 was identified in an Escherichia coli strain isolated from an outbreak of porcine diarrhoea on a farm near Nottingham, UK. The trimethoprim resistance gene did not hybridize with any of the gene probes derived from known plasmid-encoded trimethoprim resistance genes. The trimethoprim resistance gene of pUN835 was shown to encode the production of a dihydrofolate reductase which confers high-level resistance on its host. This enzyme was smaller than most plasmid-encoded dihydrofolate reductases (molecular mass = 11,500) and was labile to heat. It had relatively low affinity for the substrate dihydrofolate (Km = 20 microM) and it was resistant to competitive inhibition by trimethoprim (Ki = 7.0 microM). We classify this novel enzyme as type VII.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-7453
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-9
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:2676936-Animals,
pubmed-meshheading:2676936-DNA Probes,
pubmed-meshheading:2676936-Diarrhea,
pubmed-meshheading:2676936-Escherichia coli,
pubmed-meshheading:2676936-Escherichia coli Infections,
pubmed-meshheading:2676936-Isoelectric Focusing,
pubmed-meshheading:2676936-Nucleic Acid Hybridization,
pubmed-meshheading:2676936-R Factors,
pubmed-meshheading:2676936-Swine,
pubmed-meshheading:2676936-Swine Diseases,
pubmed-meshheading:2676936-Tetrahydrofolate Dehydrogenase,
pubmed-meshheading:2676936-Trimethoprim Resistance
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pubmed:year |
1989
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pubmed:articleTitle |
The type VII dihydrofolate reductase: a novel plasmid-encoded trimethoprim-resistant enzyme from gram-negative bacteria isolated in Britain.
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pubmed:affiliation |
Department of Bacteriology, Medical School, University of Edinburgh, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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