rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
1989-11-9
|
pubmed:abstractText |
The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced mature form of G4-forming amylase contains 530 amino acid residues with a calculated molecular mass of 57 740 Da. Sequence similarities between the G4-forming amylase and other amylolytic enzymes of species ranging from prokaryotes to eukaryotes are quite limited. However, three regions, which are involved in both the catalytic and substrate-binding sites of various amylolytic enzymes, are highly conserved in the G4-forming amylase of P. saccharophila.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0014-5793
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
11
|
pubmed:volume |
255
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
37-41
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2676600-Amino Acid Sequence,
pubmed-meshheading:2676600-Amino Acids,
pubmed-meshheading:2676600-Base Sequence,
pubmed-meshheading:2676600-Cloning, Molecular,
pubmed-meshheading:2676600-Genes, Bacterial,
pubmed-meshheading:2676600-Genetic Code,
pubmed-meshheading:2676600-Hydrolysis,
pubmed-meshheading:2676600-Molecular Sequence Data,
pubmed-meshheading:2676600-Peptide Fragments,
pubmed-meshheading:2676600-Protein Precursors,
pubmed-meshheading:2676600-Protein Sorting Signals,
pubmed-meshheading:2676600-Pseudomonas,
pubmed-meshheading:2676600-alpha-Glucosidases
|
pubmed:year |
1989
|
pubmed:articleTitle |
Nucleotide sequence of the maltotetraohydrolase gene from Pseudomonas saccharophila.
|
pubmed:affiliation |
Institute of Applied Biochemistry, University of Tsukuba, Japan.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|