Linked Life Data

2676325

Source:http://linkedlifedata.com/resource/pubmed/id/2676325

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-11-2
pubmed:abstractText
A new method of measuring polymorphonuclear leukocyte (PMN) microbicidal activity was developed in which single colony forming units of Staphylococcus aureus were isolated in microtiter wells with or without PMN, centrifuged, and incubated overnight. The percentage of bacteria killed was determined by comparing the number of PMN-containing wells showing bacterial growth to PMN-free controls. This percentage was positively correlated with the number of PMN in each well and was reduced if plates were not centrifuged or if cytochalasin B was added. Killing of either S. aureus or Escherichia coli was enhanced using fresh serum activated by prior exposure to large numbers of E. coli. There was no enhancement using heated serum that had been exposed to E. coli, nor was there enhancement when S. aureus was preopsonized by exposure to fresh serum. This assay circumvents problems in assessing microbicidal activity where aggregation of organisms might otherwise be an issue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0732-8893
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-203
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Neutrophil killing of single microorganisms as measured by a new method.
pubmed:affiliation
Medical Service, Veterans Administration Medical Center, Tuscon, Arizona 85723.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't