|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1989-10-16
|
|
pubmed:abstractText |
Full-length and deletion variants of the ets proto-oncogene and v-ets oncogene of E26 virus were expressed in E. coli using the previously described expression vectors pANH1, pJL6 and pJLA16. The recombinant proteins were expressed at greater than 5% of the total cellular proteins and were characterized by Western blot analysis using ets-specific antipeptide antibodies. A number of monoclonal antibodies were raised against p35, the expressed product of the partial human ets-2 proto-oncogene construct. These monoclonal antibodies are highly specific and recognize the p56 ets-2 product from several human cell lines.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0890-6467
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
61-6
|
|
pubmed:dateRevised |
2006-4-21
|
|
pubmed:meshHeading |
pubmed-meshheading:2674859-Antibodies, Monoclonal,
pubmed-meshheading:2674859-Blotting, Western,
pubmed-meshheading:2674859-Escherichia coli,
pubmed-meshheading:2674859-Genetic Vectors,
pubmed-meshheading:2674859-Humans,
pubmed-meshheading:2674859-Oncogenes,
pubmed-meshheading:2674859-Proto-Oncogene Proteins,
pubmed-meshheading:2674859-Proto-Oncogene Proteins c-ets,
pubmed-meshheading:2674859-Proto-Oncogenes,
pubmed-meshheading:2674859-Retroviridae Proteins,
pubmed-meshheading:2674859-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:2674859-Transcription Factors
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
Expression of human and viral ets genes in E. coli production of human ets-2-specific monoclonal antibodies.
|
|
pubmed:affiliation |
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
|
|
pubmed:publicationType |
Journal Article
|
