Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-9-22
pubmed:abstractText
Immunoglobulin heavy chain binding protein (BiP/GRP78) is a resident endoplasmic reticulum protein that binds tightly to a number of incompletely assembled or aberrant proteins. BiP also binds ATP and can be purified by ATP affinity chromatography. Here we show that an ATPase activity co-purifies with BiP prepared from canine pancreas. The BiP-associated ATPase has a high affinity for ATP but a low turnover number, suggesting a regulatory, rather than an enzymatic role. We also show that submicromolar levels of ATP or ADP decrease the rate of adsorption of [125I]BiP to nitrocellulose filters coated with protein or non-ionic detergents. In contrast, micromolar levels of AMP increase the rate of adsorption. Furthermore, ATP and ADP decrease the susceptibility of BiP to proteolytic degradation, whereas AMP was found to enhance degradation slightly. Adenine nucleotides may therefore induce or stabilize different conformations of BiP even when ATP hydrolysis does not occur.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-216411, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-2868889, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-2897629, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-2937542, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-2944601, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3021779, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3084497, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3087629, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3102505, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3121636, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3129663, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3757030, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-3849428, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-4033650, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-4092680, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-6104665, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-6146630, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-6146631, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-6314326, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-637870, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-6417546, http://linkedlifedata.com/resource/pubmed/commentcorrection/2670554-6656655
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1461-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Interaction of heavy chain binding protein (BiP/GRP78) with adenine nucleotides.
pubmed:affiliation
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.