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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5-6
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pubmed:dateCreated |
1989-10-6
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pubmed:abstractText |
The electrophoretic separation of DNA molecules can be controlled by the use of contour-clamped homogeneous electric fields (CHEF). This paper describes an improved CHEF apparatus with negligible distortion in the electric fields. When the electric field was periodically reoriented, DNA molecules up to 2 megabases were resolved in a highly uniform manner. Furthermore, when the field strength was changed with orientation, topological variations of conventional-sized DNA molecules were resolved.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0173-0835
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
290-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2670544-DNA,
pubmed-meshheading:2670544-DNA, Fungal,
pubmed-meshheading:2670544-DNA, Superhelical,
pubmed-meshheading:2670544-Electrochemistry,
pubmed-meshheading:2670544-Electrodes,
pubmed-meshheading:2670544-Electrophoresis,
pubmed-meshheading:2670544-Electrophoresis, Agar Gel,
pubmed-meshheading:2670544-Molecular Structure,
pubmed-meshheading:2670544-Molecular Weight,
pubmed-meshheading:2670544-Saccharomyces cerevisiae
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pubmed:articleTitle |
Pulsed field electrophoresis in contour-clamped homogeneous electric fields for the resolution of DNA by size or topology.
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pubmed:affiliation |
Department of Medicine, Stanford University, CA 94305.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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