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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1989-8-3
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pubmed:abstractText |
A 14-kilobase-pair (kbp) EcoRI DNA fragment that encodes an enzyme capable of rapid hydrolysis of N-methylcarbamate insecticides (carbofuran hydrolase) was cloned from carbofuran-degrading Achromobacter sp. strain WM111. When used to probe Southern blots containing plasmid and total DNAs from WM111, this 14-kbp fragment hybridized strongly to a 14-kbp EcoRI fragment from the greater than 100-kbp plasmid harbored by this strain but weakly to EcoRI-digested total DNA from Achromobacter sp. strain WM111, indicating that the gene for N-methylcarbamate degradation (mcd) is plasmid encoded. Further subcloning localized the mcd gene on a 3-kbp ScaI-ClaI fragment. There was little or no expression of this gene in the alternative gram-negative hosts Pseudomonas putida, Alcaligenes eutrophus, Acinetobacter calcoaceticus, and Achromobacter pestifer. Western blotting (immunoblotting) of the protein products produced by low-level expression in P. putida confirmed that this 3-kbp fragment encodes the two 70+-kilodalton protein products seen in sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified carbofuran hydrolase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-1254555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-16346975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-3080408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-3120825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-3123327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-3415221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-3606097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-377280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-3923930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-4336693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-4621687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-4712575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-5781579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-6257648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-6277849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-6290332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-6294059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-6651297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-7125648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-7260449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2661544-863855
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4038-44
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2661544-Alcaligenes,
pubmed-meshheading:2661544-Bacterial Proteins,
pubmed-meshheading:2661544-Biodegradation, Environmental,
pubmed-meshheading:2661544-Blotting, Western,
pubmed-meshheading:2661544-Carbofuran,
pubmed-meshheading:2661544-Cloning, Molecular,
pubmed-meshheading:2661544-Cosmids,
pubmed-meshheading:2661544-DNA, Recombinant,
pubmed-meshheading:2661544-Escherichia coli,
pubmed-meshheading:2661544-Genes, Bacterial,
pubmed-meshheading:2661544-Hydrolases,
pubmed-meshheading:2661544-Insecticides,
pubmed-meshheading:2661544-Nucleic Acid Hybridization,
pubmed-meshheading:2661544-Pseudomonas,
pubmed-meshheading:2661544-Restriction Mapping
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pubmed:year |
1989
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pubmed:articleTitle |
Cloning of a carbofuran hydrolase gene from Achromobacter sp. strain WM111 and its expression in gram-negative bacteria.
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pubmed:affiliation |
Pesticide Degradation Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
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pubmed:publicationType |
Journal Article
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