Linked Life Data

2659507

Source:http://linkedlifedata.com/resource/pubmed/id/2659507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-4
pubmed:dateCreated
1989-7-18
pubmed:abstractText
The present study was undertaken to elucidate the effect of bacterial lipopolysaccharides (LPS) on B lymphocyte derived hematopoietic growth factors. Bone marrow-derived (BM) B cells and homogeneous B cell lines (lymphomas and hybridomas) were used to study the production of granulocyte-macrophage colony stimulating factor (GM-CSF). The BM B cells required two signals for GM-CSF production: LPS and a tumor promoting phorbol ester (TPA). In the B cell lines, GM-CSF production was inducible with LPS alone. Molecular analysis of the GM-CSF gene expression revealed that steady state levels (as determined by Northern analysis) of GM-CSF mRNA could be detected only in LPS-induced cells, whereas it was found by nuclear transcription analysis that the GM-CSF gene is transcribed even in uninduced cells. However, biological activity of GM-CSF was detected only in supernatants from LPS-induced cells. These data suggest a post-transcriptional regulation of this gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0882-0139
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
B lymphocyte derived hematopoietic growth factors.
pubmed:affiliation
Laboratory of Immunology, National Institute of Dental Research, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, In Vitro