| pubmed-article:2658973 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C0007102 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C0026682 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2658973 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2658973 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2658973 | pubmed:dateCreated | 1989-6-27 | lld:pubmed |
| pubmed-article:2658973 | pubmed:abstractText | A stably differentiated clonal derivative (Cl.16E) of the human colonic adenocarcinoma cell line HT29 secretes in culture high-Mr glycoproteins that were purified from the serum-free conditioned medium by preparative SDS/polyacrylamide-gel electrophoresis. Analysis of the oligosaccharides released from the [3H]glucosamine-labelled high-Mr glycoproteins by alkaline-borohydride treatment showed that this material consisted of O-linked oligosaccharides (without any detectable N-linked oligosaccharides) that were eluted as three fractions from Bio-Gel P-6 columns. The main oligosaccharide fraction obtained after such treatment and desialylation was eluted together with a six-unit glucose polymer from a Bio-Gel P-4 column. Polyclonal antibodies were raised against the high-Mr glycoproteins, and in immunoblot analysis they reacted specifically with the high-Mr glycoproteins present in the conditioned medium. Furthermore, immunohistochemical staining of sections in paraffin wax revealed that these antibodies labelled normal human gastrointestinal mucins. We conclude that (1) the high-Mr glycoproteins prepared by SDS/polyacrylamide-gel electrophoresis are pure mucus glycoproteins on the basis of sensitivity to alkaline-borohydride treatment, monosaccharide composition and immunochemical and immunohistological findings, and (2) these mucins have antigenic determinants in common with the normal human gastrointestinal mucins. | lld:pubmed |
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| pubmed-article:2658973 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2658973 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2658973 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2658973 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2658973 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2658973 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:AubertJJ | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:FontJJ | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:CodognoPP | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:BauvyCC | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:LaboisseC LCL | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:AugeronCC | lld:pubmed |
| pubmed-article:2658973 | pubmed:author | pubmed-author:MaoretJ JJJ | lld:pubmed |
| pubmed-article:2658973 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2658973 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2658973 | pubmed:volume | 258 | lld:pubmed |
| pubmed-article:2658973 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2658973 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2658973 | pubmed:pagination | 793-9 | lld:pubmed |
| pubmed-article:2658973 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2658973 | pubmed:meshHeading | pubmed-meshheading:2658973-... | lld:pubmed |
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| pubmed-article:2658973 | pubmed:meshHeading | pubmed-meshheading:2658973-... | lld:pubmed |
| pubmed-article:2658973 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2658973 | pubmed:articleTitle | A mucus-secreting human colonic cancer cell line. Purification and partial characterization of the secreted mucins. | lld:pubmed |
| pubmed-article:2658973 | pubmed:affiliation | Laboratoire de Biologie et Physiologie des Cellules Digestives, Unité 239 I.N.S.E.R.M., Faculté de Médecine Bichat, Paris, France. | lld:pubmed |
| pubmed-article:2658973 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2658973 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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