|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1989-6-27
|
|
pubmed:abstractText |
A stably differentiated clonal derivative (Cl.16E) of the human colonic adenocarcinoma cell line HT29 secretes in culture high-Mr glycoproteins that were purified from the serum-free conditioned medium by preparative SDS/polyacrylamide-gel electrophoresis. Analysis of the oligosaccharides released from the [3H]glucosamine-labelled high-Mr glycoproteins by alkaline-borohydride treatment showed that this material consisted of O-linked oligosaccharides (without any detectable N-linked oligosaccharides) that were eluted as three fractions from Bio-Gel P-6 columns. The main oligosaccharide fraction obtained after such treatment and desialylation was eluted together with a six-unit glucose polymer from a Bio-Gel P-4 column. Polyclonal antibodies were raised against the high-Mr glycoproteins, and in immunoblot analysis they reacted specifically with the high-Mr glycoproteins present in the conditioned medium. Furthermore, immunohistochemical staining of sections in paraffin wax revealed that these antibodies labelled normal human gastrointestinal mucins. We conclude that (1) the high-Mr glycoproteins prepared by SDS/polyacrylamide-gel electrophoresis are pure mucus glycoproteins on the basis of sensitivity to alkaline-borohydride treatment, monosaccharide composition and immunochemical and immunohistological findings, and (2) these mucins have antigenic determinants in common with the normal human gastrointestinal mucins.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-1262041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-13672998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-14775715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-186353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-2965937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3311021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3328728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3360773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3567899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3597283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3611350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3756919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3857372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3896779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-3897250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-4008490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-4053024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-4066681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-4144565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-4852174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-5637714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-6199017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-6476109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-65285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-6619131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-6744312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-6746117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-6847606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-697737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-7098923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-7306054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-7430096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-865081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658973-99447
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
258
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
793-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
1989
|
|
pubmed:articleTitle |
A mucus-secreting human colonic cancer cell line. Purification and partial characterization of the secreted mucins.
|
|
pubmed:affiliation |
Laboratoire de Biologie et Physiologie des Cellules Digestives, Unité 239 I.N.S.E.R.M., Faculté de Médecine Bichat, Paris, France.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
