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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-7-5
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pubmed:abstractText |
The termini of Saccharomyces cerevisiae chromosomes consist of tracts of C1-3A (one to three cytosine and one adenine residue) sequences of approximately 450 base pairs in length. To gain insights into trans-acting factors at telomeres, high-copy-number linear and circular plasmids containing tracts of C1-3A sequences were introduced into S. cerevisiae. We devised a novel system to distinguish by color colonies that maintained the vector at 1 to 5, 20 to 50, and 100 to 400 copies per cell and used it to change the amount of telomeric DNA sequences per cell. An increase in the number of C1-3A sequences caused an increase in the length of telomeric C1-3A repeats that was proportional to plasmid copy number. Our data suggest that telomere growth is inhibited by a limiting factor(s) that specifically recognizes C1-3A sequences and that this factor can be effectively competed for by long tracts of C1-3A sequences at telomeres or on circular plasmids. Telomeres without this factor are exposed to processes that serve to lengthen chromosome ends.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-1108011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-2982162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3070325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3094961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3123310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3123321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3136437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3275867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3277178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3280967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3299061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3315231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3319189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3349525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3413114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3513174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3520552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3537715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3550434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-357984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-375221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3881185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3889913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3893744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-3907856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6091913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6246361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6248420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6256646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6286143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6287231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6310335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6313610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6324194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6330571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6344082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6345000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6361000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6377091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6383193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6403872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6765600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657397-6835846
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1488-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation.
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pubmed:affiliation |
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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