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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1989-6-27
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pubmed:abstractText |
It is generally believed that the gag gene product of human immunodeficiency virus type 1 (HIV-1) is processed into several core proteins by a virus-specific protease. We used deletion mutation analysis to study the role of HIV-specific protease in the processing of core proteins and its requirement for viral infectivity. Several mutant genomes with deletions in the protease gene were constructed. A mammalian cell line, COS-M6, transfected with the wild-type viral genome was shown to produce virions containing processed core proteins, while COS-M6 cells transfected with two mutated genomes could express only the core protein precursor, Pr56gag. The wild-type transfectant produced infectious virus; both transfectants expressing the mutated genomes also produced virions, and one of them still retained reverse transcriptase activity. However, the mutant viral particles were devoid of infectivity. Virions with a distinct central core and an electron-dense nucleoid budded out from the plasma membrane of COS-M6 cells transfected with the wild-type genome. In contrast, noninfectious virions that budded either into cytoplasmic vacuoles or out from the plasma membrane of COS-M6 cells transfected with mutant genomes contained ring-shaped nucleoids. These results indicate that the HIV-1 protease plays a role not only in the maturation of the core proteins but also in the assembly of the virus and thus is required for viral infectivity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2411050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2416054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2416713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2418504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2419593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2420008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2436298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2442162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2447506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2450209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2461297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2578227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2578615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2821409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2891772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2981635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2981931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2982104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2991655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-2992418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3027377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3035577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3040055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3258626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3260631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3282230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3290901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3292793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3302723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3306411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3321060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3474300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3643678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-3648096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-6095449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-6192195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-6328660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-6328663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2657099-6589587
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2550-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2657099-Amino Acid Sequence,
pubmed-meshheading:2657099-Animals,
pubmed-meshheading:2657099-Base Sequence,
pubmed-meshheading:2657099-Cell Line,
pubmed-meshheading:2657099-DNA, Viral,
pubmed-meshheading:2657099-Endopeptidases,
pubmed-meshheading:2657099-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2657099-Gene Expression Regulation,
pubmed-meshheading:2657099-Genes, Viral,
pubmed-meshheading:2657099-HIV Protease,
pubmed-meshheading:2657099-HIV-1,
pubmed-meshheading:2657099-Humans,
pubmed-meshheading:2657099-Immunoblotting,
pubmed-meshheading:2657099-Microscopy, Electron,
pubmed-meshheading:2657099-Molecular Sequence Data,
pubmed-meshheading:2657099-Mutation,
pubmed-meshheading:2657099-Transfection,
pubmed-meshheading:2657099-Viral Core Proteins,
pubmed-meshheading:2657099-Virion
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pubmed:year |
1989
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pubmed:articleTitle |
Role of human immunodeficiency virus type 1-specific protease in core protein maturation and viral infectivity.
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pubmed:affiliation |
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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