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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1977-5-12
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pubmed:abstractText |
Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extrachloroplastic form of S and that the small peptide portion plays a role in the transfer of S into the chloroplast.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1056023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1061088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1092698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1150747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1252082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1252083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-1256580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-164179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-4379719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-4429345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-4698907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-4748357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-4857855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-5017249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-6064364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-811671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-811672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/265554-965389
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1082-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1977
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pubmed:articleTitle |
In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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