Linked Life Data

2649503

Source:http://linkedlifedata.com/resource/pubmed/id/2649503

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1989-5-18
pubmed:abstractText
On platelets and other cell types, VLA-6 is a typical integrin heterodimer, with alpha 6-beta 1 subunit association. However, on colon carcinoma cell lines and other epithelial cells the alpha 6 subunit associates with a novel protein (called beta 4) rather than the VLA beta 1 subunit. The beta 4 protein differs from beta 1 because (i) it is not recognized by anti-beta 1 antibodies, (ii) it yields different V8 protease cleavage products, (iii) it has a more limited cell distribution, (iv) it has multiple forms, each larger in size than beta 1, and (v) it is susceptible to protease digestion which does not effect beta 1. Although different in many respects, the beta 4 subunit does have partial N-terminal sequence similarity to the already defined integrin beta 1, beta 2, and beta 3 subunits. The presence of alpha 6-beta 4 complexes was demonstrated by coprecipitation of beta 4 with an anti-alpha 6 antibody and by covalent cross-linking experiments. Although alpha 6-beta 4 complexes were present on certain cells, other VLA alpha subunits on those same cells remained associated with the VLA beta 1 subunit to form typical VLA heterodimers (e.g. VLA-1, VLA-2, VLA-3). By the criteria of N-terminal amino acid sequencing, antibody recognition, V8 peptide maps, and reduced/nonreduced gel migration, the alpha 6 subunit which associates with beta 4 appears identical to the alpha 6 associated with the VLA beta 1 subunit on platelets and other cell types. The beta 4 subunit may be of major importance because (i) it is highly abundant on the surface of colon carcinoma cell lines, and (ii) it is highly immunogenic relative to other surface proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6529-35
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines.
pubmed:affiliation
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.