Linked Life Data

2649250

Source:http://linkedlifedata.com/resource/pubmed/id/2649250

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-5-19
pubmed:databankReference
pubmed:abstractText
The yeast tropomyosin gene, designated TPM1, is present in a single copy per haploid genome and encodes a protein with a predicted molecular weight of 23.5 kd. The protein sequence is homologous to higher cell tropomyosins, including the characteristic hydrophobic-hydrophilic pseudoheptapeptide repeats. Indirect immunofluorescence microscopy reveals that tropomyosin is localized with actin cables in wild-type cells. Disruption of TPM1 is not lethal, but results in a reduced growth rate and disappearance of actin cables. Strains carrying the conditional actin mutation act1-2 also lack actin cables; overexpression of tropomyosin in these strains partially restores actin cables. These results strongly suggest that tropomyosin interacts with F actin in vivo and may play an important role in assembling or stabilizing actin cables in yeast.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton.
pubmed:affiliation
Section on Biochemistry Molecular, Cornell University, Ithaca, New York 14853.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.