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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-4-12
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pubmed:abstractText |
We report in this paper on isolation and characterization of two unknown nucleosides G* and [A*] located in the T-psi-stem of yeast methionine initiator tRNA, using the combined means of HPLC protocols, real time UV-absorption spectrum, and post-run mass spectrometry by electron impact or fast atom bombardment. The G* nucleoside in position 65 was identified as unmodified guanosine. The structure of the unknown [A*] in position 64 was characterized as an isomeric form of O-ribosyl-adenosine by comparison of its chromatographic, UV-spectral and mass spectrometric properties with those of authentic O-alpha-ribofuranosyl-(1"----2')-adenosine isolated from biosynthetic poly(adenosine diphosphate ribose). Our studies also brought evidence for the presence of a phosphorylmonoester group located on this new modified nucleoside [A*], when isolated by ion exchange chromatography from enzymic hydrolysis of yeast initiator tRNAMet without phosphatase treatment.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-14323568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-218210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-4344891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-4349570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-4350482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-5105575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-5582428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-6184222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-632336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-6370316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-6436645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-6766719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-6782097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-6844697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-7050138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-856843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2646591-956312
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleosides,
http://linkedlifedata.com/resource/pubmed/chemical/Poly Adenosine Diphosphate Ribose,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Amino Acid-Specific,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Met
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
865-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2646591-Adenosine,
pubmed-meshheading:2646591-Chromatography, DEAE-Cellulose,
pubmed-meshheading:2646591-Chromatography, High Pressure Liquid,
pubmed-meshheading:2646591-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:2646591-Guanosine,
pubmed-meshheading:2646591-Methionine,
pubmed-meshheading:2646591-Nucleic Acid Conformation,
pubmed-meshheading:2646591-Nucleosides,
pubmed-meshheading:2646591-Phosphorylation,
pubmed-meshheading:2646591-Poly Adenosine Diphosphate Ribose,
pubmed-meshheading:2646591-RNA, Transfer, Amino Acid-Specific,
pubmed-meshheading:2646591-RNA, Transfer, Met,
pubmed-meshheading:2646591-Saccharomyces cerevisiae
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pubmed:year |
1989
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pubmed:articleTitle |
Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.
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pubmed:affiliation |
Laboratoire de Biochimie Médicale, Université de Bourgogne, Dijon, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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