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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1989-4-6
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pubmed:abstractText |
We recently observed a 25-fold increase in the activity of ornithine decarboxylase (ODC) 6 h after treatment of G1-synchronized CTLL-2 cells with interleukin-2 (IL-2). Here we show that the ODC mRNA content increased in parallel with the ODC activity during the first hours of stimulation with IL-2, resulting in a 25-fold increase at 6 h. Between 6 and 24 h the ODC mRNA content continued to increase steadily up to 50-fold, even after the ODC activity had returned to low basal levels. In the case of density-arrested CTLL-2 cells deprived of IL-2 for 16 h, the IL-2-mediated increase in ODC mRNA was 2-fold at 1 h and 5-fold at 8 h, irrespective of the capability of the cells to resume their cycle. There was no marked increase in the rate of transcription of the ODC gene, at least during the first 2 h of stimulation with IL-2. These findings suggest that the regulation of the ODC activity by IL-2 is a primary event in IL-2-induced cell proliferation and occurs at the post-transcriptional level, possibly by stabilizing the ODC mRNA and affecting the efficiency of translation of the messenger.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-4827
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
181
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
273-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2645150-Animals,
pubmed-meshheading:2645150-Cell Count,
pubmed-meshheading:2645150-Cell Division,
pubmed-meshheading:2645150-Clone Cells,
pubmed-meshheading:2645150-Gene Expression Regulation,
pubmed-meshheading:2645150-Interleukin-2,
pubmed-meshheading:2645150-Mice,
pubmed-meshheading:2645150-Ornithine Decarboxylase,
pubmed-meshheading:2645150-Proto-Oncogene Proteins,
pubmed-meshheading:2645150-Proto-Oncogenes,
pubmed-meshheading:2645150-RNA, Messenger,
pubmed-meshheading:2645150-T-Lymphocytes,
pubmed-meshheading:2645150-Transcription, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
Interleukin-2 induces a rapid increase in ornithine decarboxylase mRNA in a cloned murine T lymphocytic cell line.
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pubmed:affiliation |
Department of Pathology, University of Helsinki, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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