Linked Life Data

2644285

Source:http://linkedlifedata.com/resource/pubmed/id/2644285

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1989-3-21
pubmed:abstractText
A bacterial expression system for the parvalbumin-like calcium-binding protein oncomodulin has been constructed. This system can yield 50-fold more oncomodulin than the richest known mammalian source, the rat Morris hepatoma 5123. The bacterially produced protein folded correctly as monitored by UV, fluorescence, and 1H nuclear magnetic resonance spectroscopy and is immunologically identical to rat hepatoma oncomodulin. A calcium-specific conformational change is observed in the bacterial oncomodulin similar to that of the hepatoma protein. A modification of the putative calcium-specific CD loop by site-directed mutagenesis, which changed Asp-59 to Glu-59, eliminates calcium-specific effects. In contrast to the native molecule, the mutant Glu-59 now exhibits a magnesium-induced conformational change when monitored by UV difference or fluorescence excitation spectroscopy. The availability of large amounts of bacterially produced oncomodulin combined with the ability to modify at will the metal-binding ligands should now allow dissection of the unusual pairing in oncomodulin of one calcium-specific calmodulin-like site with one calcium/magnesium parvalbumin-like site.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3470-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:2644285-Amino Acid Sequence, pubmed-meshheading:2644285-Binding Sites, pubmed-meshheading:2644285-Calcium, pubmed-meshheading:2644285-Calcium-Binding Proteins, pubmed-meshheading:2644285-DNA, Recombinant, pubmed-meshheading:2644285-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:2644285-Escherichia coli, pubmed-meshheading:2644285-Gene Expression Regulation, pubmed-meshheading:2644285-Magnesium, pubmed-meshheading:2644285-Magnetic Resonance Spectroscopy, pubmed-meshheading:2644285-Molecular Sequence Data, pubmed-meshheading:2644285-Mutation, pubmed-meshheading:2644285-Neoplasm Proteins, pubmed-meshheading:2644285-Plasmids, pubmed-meshheading:2644285-Protein Conformation, pubmed-meshheading:2644285-Spectrometry, Fluorescence, pubmed-meshheading:2644285-Spectrophotometry, Ultraviolet
pubmed:year
1989
pubmed:articleTitle
Characterization and site-specific mutagenesis of the calcium-binding protein oncomodulin produced by recombinant bacteria.
pubmed:affiliation
Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.
pubmed:publicationType
Journal Article, Comparative Study