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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1978-8-28
|
| pubmed:abstractText |
A fraction of rat liver polyribosomes is isolated, which in its immunochemical characteristics considerably enriched with polyribosomes capable to synthesize hydrocortisone-induced liver tyrosine aminotransferase isoenzyme. This specific polyribosome fraction was purified by immunochemical fractionation of total liver polyribosomes using indirect precipitation. The content of polyribosomes in immunoprecipitates comprise 0.4-0.8% of its initial amount (before immunochemical fractionation). The ratio of specific polyribosomes in immunoprecipitates varies from 20 to 45%, which corresponds to 25-100-fold purification. The data obtained suggest that the method of indirect precipitation can be an efficient step in the isolation procedure of individual mRNA.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0320-9725
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
625-30
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:26425-Animals,
pubmed-meshheading:26425-Antibodies,
pubmed-meshheading:26425-Cell Fractionation,
pubmed-meshheading:26425-Immunochemistry,
pubmed-meshheading:26425-Liver,
pubmed-meshheading:26425-Polyribosomes,
pubmed-meshheading:26425-Precipitin Tests,
pubmed-meshheading:26425-RNA, Messenger,
pubmed-meshheading:26425-Rats,
pubmed-meshheading:26425-Tyrosine Transaminase
|
| pubmed:year |
1978
|
| pubmed:articleTitle |
[Indirect immunoprecipitation by rat liver polyribosomes using antibodies to tyrosine aminotransferase].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|