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rdf:type |
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lifeskim:mentions |
umls-concept:C0009219,
umls-concept:C0019932,
umls-concept:C0026336,
umls-concept:C0027895,
umls-concept:C0035696,
umls-concept:C0037378,
umls-concept:C0162788,
umls-concept:C0323913,
umls-concept:C0337048,
umls-concept:C0567416,
umls-concept:C1511790,
umls-concept:C1514697,
umls-concept:C2603343
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pubmed:issue |
1
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pubmed:dateCreated |
1989-1-24
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pubmed:abstractText |
To develop and optimize non-radioactive in situ hybridization techniques for mRNA detection, we used the neuropeptidergic system of the pond snail Lymnaea stagnalis as a biological model system. First, we investigated the in situ hybridization procedure using radioactive-labeled cDNA and synthetic oligonucleotide probes specific for egg-laying hormone (ELH) mRNA and molluscan insulin-like peptide (MIP) mRNA. The results show an intense grain deposit above the caudodorsal cells and light-green cells expressing, respectively, ELH mRNA and MIP mRNA. Good results with relation to signal strength and tissue morphology were obtained with freeze-dry paraformaldehyde vapor fixation. The necessity to perform tissue pre-treatment appeared to be dependent on the cell type of interest. The optimized in situ hybridization protocol proved to be applicable using probes that are either sulfonated/transaminated or labeled with acetylaminofluorene (AAF). In situ hybridization of such haptenized probes led to intense and specific staining of the cytoplasm of the caudodorsal cells. Egg-laying hormone mRNA appeared not to be homogeneously distributed in the cytoplasm but showed a "patch-like" pattern. Nuclear and axoplasmic staining for mRNA was also observed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Acetylaminofluorene,
http://linkedlifedata.com/resource/pubmed/chemical/3,3'-Diaminobenzidine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Invertebrate Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/egg-laying hormone, Mollusca
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1554
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-14
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2642295-2-Acetylaminofluorene,
pubmed-meshheading:2642295-3,3'-Diaminobenzidine,
pubmed-meshheading:2642295-Animals,
pubmed-meshheading:2642295-DNA Probes,
pubmed-meshheading:2642295-Immunohistochemistry,
pubmed-meshheading:2642295-Insulin,
pubmed-meshheading:2642295-Invertebrate Hormones,
pubmed-meshheading:2642295-Lymnaea,
pubmed-meshheading:2642295-Neuropeptides,
pubmed-meshheading:2642295-Nucleic Acid Hybridization,
pubmed-meshheading:2642295-Oligonucleotide Probes,
pubmed-meshheading:2642295-Peptides,
pubmed-meshheading:2642295-Phosphorus Radioisotopes,
pubmed-meshheading:2642295-RNA, Messenger,
pubmed-meshheading:2642295-Sulfur Radioisotopes
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pubmed:year |
1989
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pubmed:articleTitle |
Detection of mRNA molecules coding for neuropeptide hormones of the pond snail Lymnaea stagnalis by radioactive and non-radioactive in situ hybridization: a model study for mRNA detection.
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pubmed:affiliation |
Department of Cytochemistry and Cytometry, University of Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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