Linked Life Data

2631797

Source:http://linkedlifedata.com/resource/pubmed/id/2631797

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-5-14
pubmed:abstractText
Several of the autoimmune defects of NZB mice have been linked to chromosome 4 where the Lps gene which regulates B cell activation by bacterial lipopolysaccharide (LPS) is found. Thus, studies of an NZB.Lpsd strain may facilitate functional analysis of B cell hyperactivity. To develop NZB.Lpsd mice, the Lpsd mutation of C57BL/10ScN mice was further characterized by studying the influence of Lpsd on LPS-induced spleen cell proliferation colony-stimulating factor (CSF) production, and B cell colony-forming unit (CFU-B) proliferation in (C57BL/10SnJ X C57BL/10ScN) F1 X C57BL/10ScN mice. Twenty-one of 27 backcross offspring demonstrated concordance of results in the three assays indicating common genetic regulation of these traits. Subsequently, the Lps allele of NZB mice was characterized by determining the mitogen responsiveness, CSF production and CFU-B proliferation of (NZB X C57BL/10ScN) F1 X C57BL/10ScN mice. In addition, concordance of assortment of the C57BL/10ScN Mupb allele and LPS unresponsiveness was verified. Results of these assays were concordant in 12 of 14 backcross mice, indicating that NZB LPS responsiveness is also regulated by a gene or closely linked set of genes on chromosome 4. Further, the LPS responsiveness of homozygous fifth backcross NZB.Lpsd mice was significantly diminished compared to that of NZB mice. Interestingly, the hypergammaglobulinemia and anti-DNA antibody levels in 6-month-old Lpsd mice did not differ from those of NZB mice despite the absence of LPS-responsive CFU-B.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0254-9670
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-203
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Genetic regulation of lipopolysaccharide responses in NZB mice.
pubmed:affiliation
Division of Rheumatology/Allergy and Clinical Immunology, University of California, Davis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.