Linked Life Data

2628171

Source:http://linkedlifedata.com/resource/pubmed/id/2628171

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-4-24
pubmed:abstractText
Previous studies on the rec1 mutant of Ustilago maydis revealed a complex phenotype. The mutant was found to be defective in DNA repair, recombination, growth, mutagenesis, and meiosis. We cloned the REC1 gene by complementing the phenotype of the mutant using a genomic DNA library made in a replicating vector and provided proof of identity by one-step gene disruption of the wild-type (wt) REC1 locus with a mutant allele constructed in vitro. The transcript of the REC1 gene was identified as a damage-inducible 1.7-kb RNA. After UV irradiation, the level of the transcript was observed to rise ten- to 15-fold. In the rec1 mutant, the basal level of the 1.7-kb transcript was tenfold lower than in wt. In addition, a second transcript of 1.1 kb was noted in rec1-1 cells in response to DNA damage.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-41
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Isolation of the REC1 gene controlling recombination in Ustilago maydis.
pubmed:affiliation
Department of Microbiology, Cornell University Medical College, New York, NY 10021.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.