Linked Life Data

2619925

Source:http://linkedlifedata.com/resource/pubmed/id/2619925

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1990-3-20
pubmed:abstractText
The permeability of liposomes prepared from beef heart mitochondrial phospholipids was studied after treatment with phospholipase A2. The permeability to H+ ions was measured by recording the rate of change of pH in the external medium following an addition of an aliquot of alkali to liposomes with a highly buffered inner medium, while the penetration of Ca2+ ions into liposomes was measured in liposomes loaded with arsenazo III. There was a doubling of H+ permeability when the lysophospholipid content was increased to 2% by treatment with phospholipase A2, and a tripling at 4%. Entrapped sucrose leakage from liposomes became apparent at above 6% lysophospholipid. Treatment with phospholipase A2 stimulated Ca2+ penetration into liposomes driven by a valinomycin-induced diffusion potential or a nigericin-induced H+ gradient. The data are discussed in relation to the mechanism of damage to mitochondria occurring in Ca2+ overload as well as in phospholipase A2-induced cellular damage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0177-3593
pubmed:author
pubmed:issnType
Print
pubmed:volume
370
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1315-20
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
The phospholipase A2-induced increase in the permeability of phospholipid membranes to Ca2+ and H+ ions.
pubmed:affiliation
Institutionen för medicinsk kemi, Helsingfors Universitet.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't