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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1978-7-15
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pubmed:abstractText |
The activity of some enzymes involved in pyrimidine nucleotide synthesis was studied in rat embryo cell (REC) cultures infected with X14 or H-1 parvovirus. dCMP aminohydrolase activity of the infected cells was 64--121% greater than that of the mock-infected cells. dTMP synthetase activity was 18% greater in X14 virus-infected cells and 34% lower in H-1 virus-infected cells. These results suggest some differences in the infected cells, as regards the biosynthesis of dTMP. Orotate phosphoribosyltransferase and orotidylate decarboxylase activities appeared nearly unmodified compared to the mock-infected cells. The addition of phosphoribosylpyrophosphate (PRPP) to the cell suspension incubated with [6-14C] orotate increased the specific radioactivity of acid-soluble uracil, 5-fold in the mock-infected cells and 15- --24-fold in the X14 or H-1 virus-infected cells (72 hr p.i.). This result suggests that the lowered pyrimidine nucleotide synthesis in infected cells depends to a large extent on the diminished PRPP pool.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carboxy-Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytosine,
http://linkedlifedata.com/resource/pubmed/chemical/DCMP Deaminase,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotide Deaminases,
http://linkedlifedata.com/resource/pubmed/chemical/Orotate Phosphoribosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Orotidine-5'-Phosphate Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Pentosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoribosyl Pyrophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidylate Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Uracil
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0001-723X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
104-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:26195-Carboxy-Lyases,
pubmed-meshheading:26195-Cell Line,
pubmed-meshheading:26195-Cytosine,
pubmed-meshheading:26195-DCMP Deaminase,
pubmed-meshheading:26195-Methyltransferases,
pubmed-meshheading:26195-Nucleotide Deaminases,
pubmed-meshheading:26195-Orotate Phosphoribosyltransferase,
pubmed-meshheading:26195-Orotidine-5'-Phosphate Decarboxylase,
pubmed-meshheading:26195-Parvoviridae,
pubmed-meshheading:26195-Pentosyltransferases,
pubmed-meshheading:26195-Phosphoribosyl Pyrophosphate,
pubmed-meshheading:26195-Pyrimidines,
pubmed-meshheading:26195-Thymidylate Synthase,
pubmed-meshheading:26195-Uracil
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pubmed:year |
1978
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pubmed:articleTitle |
Pyrimidine nucleotide synthesis in rat embryo cells infected with X14 or H-1 parvovirus.
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pubmed:publicationType |
Journal Article
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