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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1990-3-15
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pubmed:abstractText |
An immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA) for the detection of dengue antibodies is described and compared to the haemagglutination inhibition test (HI). The sensitivity, specificity and agreement rate between the 2 techniques were good. The coefficients of correlation between IgG-ELISA and HI results, using dengue 1-4 antigens, were highly significant (P less than 0.001 for either antigen). IgG-ELISA was rapid, and easy to perform and suitable for large-scale studies. Between April and June 1987, a baseline serosurvey of the prevalence of dengue antibodies in age-stratified samples of children was carried out in Tahiti using IgG-ELISA. 327 children were tested against each dengue serotype. There was no significant difference between sex. Overall rates ranged from 7.4% in children under 5 years to 83.1% in those aged 15-19 years. Dengue 4 being the only serotype involved since 1979, specific responses to dengue 4 were found in higher proportion among children under 8 years old, while the IgG-ELISA responses were more widely reactive among children aged above 8 years (P less than 0.001). The acquisition rate of dengue 4 antibodies was 2.95% on average per year in children under 8 years of age.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0035-9203
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
708-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2617636-Age Factors,
pubmed-meshheading:2617636-Antibodies, Viral,
pubmed-meshheading:2617636-Antigens, Viral,
pubmed-meshheading:2617636-Child,
pubmed-meshheading:2617636-Child, Preschool,
pubmed-meshheading:2617636-Dengue,
pubmed-meshheading:2617636-Dengue Virus,
pubmed-meshheading:2617636-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2617636-Female,
pubmed-meshheading:2617636-Hemagglutination Inhibition Tests,
pubmed-meshheading:2617636-Humans,
pubmed-meshheading:2617636-Immunoglobulin G,
pubmed-meshheading:2617636-Male,
pubmed-meshheading:2617636-Polynesia,
pubmed-meshheading:2617636-Predictive Value of Tests,
pubmed-meshheading:2617636-Prevalence,
pubmed-meshheading:2617636-Regression Analysis
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pubmed:articleTitle |
Comparison of immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA) and haemagglutination inhibition (HI) test for the detection of dengue antibodies. Prevalence of dengue IgG-ELISA antibodies in Tahiti.
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pubmed:affiliation |
Institute Territorial de Recherches Médicales Louis Malardé, Tahiti, French Polynesia.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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