Linked Life Data

2616874

Source:http://linkedlifedata.com/resource/pubmed/id/2616874

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-3-1
pubmed:abstractText
Three forms (I, II and III) of glucose-6-phosphate dehydrogenase were isolated from mycelium of Aspergillus oryzae grown on ribose as the carbon source, by ion-exchange chromatography. The Km values determined for the three forms with respect to glucose-6-phosphate were nearly identical; however the Km for NADP+ were different and the Vmax for the isoenzymatic form II was higher than those for I and III. Inhibition by NADPH was competitive with respect to NADP+, isoenzyme II showing the highest Ki. The optimum pH for forms I, II and III were 9.0, 8.0 and 8.5, respectively, and form I was more thermostable than the others. The apparent molecular weights, determined by gel filtration, were 92,000, 117,500 and 141,000 for forms I, II and III, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0034-9402
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Characterization of three enzymatic forms of glucose-6-phosphate dehydrogenase from Aspergillus oryzae.
pubmed:affiliation
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.
pubmed:publicationType
Journal Article