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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1990-3-15
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pubmed:abstractText |
Bacillus thuringiensis strain AF101 possesses a single plasmid (pAF101) with a molecular size of 42 MDa (69 kb). During plasmid curing experiments in strain AF101, we found that a phage (J7W-1) was induced by ethidium bromide treatment. Moreover, the phage genome (48 kb) hybridized only with pAF101 on a Southern blot of the DNA of a cleared lysate prepared from strain AF101. Comparison of the restriction patterns of pAF101 and J7W-1 phage DNA revealed that pAF101 contains not only the entire phage DNA but also a plasmid-specific DNA region. These results indicate that the J7W-1 genome has been stably integrated into pAF101 in strain AF101. Integration of the J7W-1 genome into a plasmid was also observed after phage infection of the type strain of B. thuringiensis subsp. israelensis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1287
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3035-41
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:2614379-Bacillus thuringiensis,
pubmed-meshheading:2614379-Bacteriophages,
pubmed-meshheading:2614379-Ethidium,
pubmed-meshheading:2614379-Genes, Viral,
pubmed-meshheading:2614379-Microscopy, Electron,
pubmed-meshheading:2614379-Molecular Weight,
pubmed-meshheading:2614379-Plasmids,
pubmed-meshheading:2614379-Virus Activation
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pubmed:year |
1989
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pubmed:articleTitle |
A novel phage genome integrated into a plasmid in Bacillus thuringiensis strain AF101.
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pubmed:affiliation |
Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article
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