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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1990-3-1
|
| pubmed:abstractText |
The RNA branch formed during pre-mRNA splicing occurs at a wide variety of sequences (branch sites) in different mammalian pre-mRNAs. U2 small nuclear ribonucleoprotein (snRNP) binds to the pre-mRNA branch site following the interaction of a protein, U2AF, with the 3' splice site/polypyrimidine tract. Here we show that despite the variability of mammalian branch sites, U2 snRNP has a sequence-specific RNA-binding activity. Thus, RNA branch formation is regulated by two sequence-specific interactions: U2AF with the 3' splice site/polypyrimidine tract, and U2 snRNP with the branch site. The affinity of the branch site for U2 snRNP affects the efficiency of spliceosome assembly and splicing.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1562-71
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2612905-Animals,
pubmed-meshheading:2612905-Base Sequence,
pubmed-meshheading:2612905-Binding Sites,
pubmed-meshheading:2612905-Humans,
pubmed-meshheading:2612905-Molecular Sequence Data,
pubmed-meshheading:2612905-RNA, Small Nuclear,
pubmed-meshheading:2612905-RNA Precursors,
pubmed-meshheading:2612905-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Mammalian U2 snRNP has a sequence-specific RNA-binding activity.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|